Systematic Reverse Genetics of Transfer-DNA-Tagged Lines of Arabidopsis
نویسندگان
چکیده
منابع مشابه
Systematic reverse genetics of transfer-DNA-tagged lines of Arabidopsis. Isolation of mutations in the cytochrome p450 gene superfamily.
We have developed an efficient reverse-genetics protocol that uses expedient pooling and hybridization strategies to identify individual transfer-DNA insertion lines from a collection of 6000 independently transformed lines in as few as 36 polymerase chain reactions. We have used this protocol to systematically isolate Arabidopsis lines containing insertional mutations in individual cytochrome ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1998
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.118.3.743